This half-hour comedy focuses on the bizarre experiences of fresh-faced medical intern John "J.D." Dorian (Zach Braff) as he embarks on his healing career in a surreal hospital crammed full of unpredictable staffers and patients – where humor and tragedy can merge paths at any time.

Joining the rumpled J.D. in his exhilarating brave new world are his college buddy, Chris Turk (Donald Faison, "Clueless"), an intern with a more elite surgical group, and J.D.'s fellow medical intern, the beautiful and driven Elliot Reid (Sarah Chalke, "Roseanne"). Keeping the new interns on their toes are: The fatherly chief of medicine, Dr. Bob Kelso (Ken Jenkins); the abrasive, worldly Dr. Perry Cox (John McGinley), and the caring but slightly jaded nurse Carla Espinosa (Judy Reyes). The hospital janitor (Neil Flynn) also never seems to miss an opportunity to harass his target.

Scrubs is filmed in a real-life hospital. It's the North Hollywood Medical Center, which has gone under severe reconstruction to fit all the equipment and represent Sacred Heart Hospital. Interesting trivia: Sacred Heart was the name of the school that Christa Miller worked; in one episode Zach Braff accidentally calls the Janitor Neil Flynn, which is the actor's real life name.

Dr. John Michael Dorian, more commonly known as J.D. is an internist (Doctor of Internal Medicine) at Sacred Heart Hospital. He is currently in his seventh year there, where was an intern for one year, a resident for three years, and is currently an attending physician in internal medicine. J.D. is in his 30s and currently is homeless, but lives in with his best friend/roommate. He is in a relationship with Kim, Kim currently has had the baby who is named Sam Dorian after J.D.'s dad. J.D. has several oddities about him, such as how he fantasizes everyday, therefore finding humour and hope in every situation. He also narrates his own life.

J.D.'s parents are Sam Dorian and Barbara Dorian, now Barbara Turner. He has one older brother, Dan. Sam and Barbara are divorced, and Barbara has married six times since their divorce. Sam has recently died. Other members of J.D.'s family are Grandma Dorian and Nana Hobbs.

At Sacred Heart, J.D. has served in various positions and roles, aside from being a practicing doctor. He served as Chief Resident with Elliot Reid for his fourth year at Sacred Heart, of which the Janitor convinced everyone to call him Co-Chief. He also works as staff internist, from the end of his fourth year to the present. He has not chosen his specialty, deciding between Oncology and Radiology. J.D. is a legitimately good doctor, who honestly cares for his patients.

John Michael "J.D." Dorian
Elliot is the daughter of Simon and Lily Reid. Elliot grew up in a very privileged life, but her parents were never around to love her. She was raised by her housemaid Consuela. She claims that she was named "Elliot" because her parents were expecting a boy. Elliot has four brothers who are all doctors. Known are Barry, who is gay, and Bradley. She also has a Great Aunt Sally and a Great Uncle whom J.D. has met. Her second year at Sacred Heart, Elliot refused to follow the specialty her father desired, and he cut her off finacially. She has also showed Dr. Kelso that she refuses to follow typical female doctors who end up in family practice or as maternity doctors.

At Sacred Heart Hospital, Elliot began as an intern and later became a resident. She served as Chief Resident with J.D., but was not forced to be called "Co-Chief" as he was. At the end of her fourth year at Sacred Heart, she briefly left to take on an endocrinology fellowship at North County University Medical Center, which lasted only 5 days. She returned to Sacred Heart and became a senior attending physician, but soon entered private practice. Dr. Cox and Dr. Kelso are upset that she is in private practice, but she stands by her decision.

Elliot often tells long, seemingly pointless stories and has several quirks about her. For example, her voice gets really high pitched and squeaky when she gets upset. She also fantasizes about old men and cider houses. Elliot refused to say certain body organs or sex terms, and refers to them as "bajingos," "hoo hoos," and "intercourse".

Dr. Elliot Reid
Dr. Cox is a senior attending physician at Sacred Heart Hospital and Residency Director for the hospital. Cox is around 44 years old, and of Irish descent. He is the sarcastic, bitter mentor of J.D.. Dr. Cox routinely rants at and belittles J.D., though his apparently rough treatment of J.D. is intended as conditioning for the rigors and horrors of hospital life, as well as an outlet for Dr. Cox's frustration in his personal life. He is secretly proud of J.D. and thinks he has the potential to become an incredible doctor.

Cox's family is from Pittsburgh. He has a sister Paige, who is a born-again Christian. His father was an abusive alcoholic and his mother did nothing to prevent this behavior. When questioned why his sister is so religious, he says "maybe the TV was broken so she picked up a Bible and found it to be just a darn good read or maybe it has something to do with our mother's ability to watch silently as our dad drunkenly knocked us from room to room." Perry's abusive and traumatic childhood could help explain his intimacy issues and his marriage to Jordan. He and Paige have a hostile and difficult relationship, ostensibly because neither can appreciate the religious and scientific viewpoints held by the other, but it was ultimately revealed that Paige's presence reminded Cox of the troubled childhood that he consistently tried to forget.

Cox was also best friend's with Jordan's brother, Ben, and was very shocked when he found out Ben had leukemia, and very saddened when Ben dies.

Dr. Cox has two children by Jordan - a son, Jack, and a daughter, Jennifer Dillon. He has had a vasectomy, reversed it and then had another vasectomy, but it didn't take.

John Michael "J.D." Dorian
Turk has been at Sacred Heart for six years, alongside J.D. and Elliot. He has been J.D.'s best friend since they were college roommates. He is married to Carla, and they have one daughter, Isabella Turk. Although he is a surgeon, Turk does not fully embrace the "jock-like" status of one, such as The Todd. He has been diagnosed with Type 2 Diabetes. He does enjoy playing basketball in the parking lot, however. Turk strives to be the best in all that he does at work, at being a friend, and at being a family member to his wife, daughter or immediate family. His immediate family includes his mother Margaret Turk, brother Kevin Turk, Jabbari Turk, cousins Nfume Turk, George Turk, and Shedaisy Turk. Turk has lived with J.D. and Rowdy, but currently lives with Carla and their daughter in the apartment.
Christopher "Chris" Duncan Turk
Carla, like most, made her first appearance in the first episode, My First Day. J.D. froze during his first operation, but Carla stepped in to assist him. She basically plays the role that is standard for nurses in Scrubs; assisting the otherwise clueless doctors. Her serious nature makes her stand out among the other three main characters, (J.D., Elliot, and Turk.) who are usually silly and quirky. Unlike them, she is very rarely embarassed, out-smarted, or made to look like a fool, though she has been called out for being nosy, bossy, and for lacking a sense of humor about herself. Often times, she stands up for her friends, and says things they're afraid to say to Dr. Kelso and Dr. Cox, especially with J.D, who she nicknamed "Bambi", derived from the timid way he came into Sacred Heart. Quickly, she got into a relationship with Turk, which still stands today. At first, she labeled him as a normal idiot, that just wanted a hot nurse, but eventually, he convinced her to give him a chance. Soon after, they started dating, and got married.
Nurse Carla Espinosa
Janitor takes a great pleasure in making Jonathan Dorian's life a living hell. As of Season Five he has worked at Sacred Heart for 12 years, but has revealed that he is 42 years old. He is a wonderful liar and story teller, and can get nearly anyone to believe anything he says, even if they know of his tendency towards dissembling. He fools people into believing a variety of crazy stories, some of which turn out to be true. According to himself, he was once a world-class 100 meter hurdler which he proved.

The Janitor has many strange hobbies and skills, and one of the biggest is taxidermy. This is especially true when it comes to squirrels, as he is shown to have a gigantic squirrel army at one point in the series. As for his family, he claims he is married to a woman with a few finger digits, pointer on one hand and thumb/pinky on the other. He has no children, but has often joked that he is J.D.'s father. He does have a father who other hospital staff members have seen. In the custodial society of Sacred Heart, he is seen as a leader and the other custodians seem to obey his every command. He only appears to fear Nurse Carla Espinosa, Turk's wife.

In the episode My Identity Crisis, J.D.has to learn everyone in the hospitals name as a bet to The Janitor, which he thinks he does. But then Janitor puts a picture of himself up and J.D. says, "No one knows your name".

The Janitor